ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Apis mellifera (Honeybee)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
59.39 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Major royal jelly protein 1, MRJP-1, MRJP1, 56-kDa protein 4, p56kP-4, Apalbumin 1, Apisin subunit MRJP1, Bee-milk protein, Royalactin, Jellein-1, Jelleine-I, Jellein-2, Jelleine-II, Jellein-4, Jelleine-IV, MRJP1
Background
Major royal jelly protein 1 (Api m 11/MRJP1) is a ~48 kDa protein. Most abundant protein component of royal jelly, a substance produced in the hypopharyngeal gland containing proteins, free amino acids, fatty acids, sugars and other nutrients, which is fed to developing larvae by worker nurse bees. Major royal jelly proteins (Mrjps) are high in essential amino acids and probably have a nutritional function in larval food. All larvae are fed some royal jelly (also known as worker jelly) early in their development but it forms the principal source of nutrition for larvae destined to become queen bees (Probable). Induces the differentiation of honey bee larvae into queens through an Egfr-mediated signaling pathway. Promotes body size increase by activating p70 S6 kinase, stimulates ovary development by augmenting the titer of vitellogenin (Vg) and juvenile hormone, and reduces developmental time by increasing the activity of mitogen-activated protein kinase and inducing 20-hydroxyecdysone (ecdysterone, 20E) production.
1. Santos, KS. et al. (2005) Insect biochemistry and molecular biology 35, 85-91. PMID: 15607658
2. Tamura, S. et al. (2009) Proteomics 9, 5534-43. PMID: 20017154
3. Kamakura, M. (2011) Nature 473, 478-83. PMID: 21516106
4. Dobritzsch, D. et al. (2019) Ecology and evolution 9, 8771-8782. PMID: 31410279
5. Schmitzová, J. et al. (1998) Cellular and molecular life sciences : CMLS 54, 1020-30. PMID: 9791542
6. Buttstedt, A. et al. (2018) Current biology : CB 28, 1095-1100.e3. PMID: 29551410
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Honeybee Api m 11/MRJP1 Protein, N-His-SUMO
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