Recombinant HRSV Nucleoprotein, N-His (VK088012)

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Overview

Catalog No.	VK088012
Description	Recombinant HRSV Nucleoprotein, N-His (VK088012) expressed in E. coli, spanning Met1-Leu391. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P03418
Protein length	Met1-Leu391
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human respiratory syncytial virus
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	45.61 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Nucleoprotein, Protein N, Nucleocapsid protein, N
Background	Nucleoprotein is a ~43 kDa protein. Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. RNA replication depends on the availability of soluble nucleoprotein. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Together with the phosphoprotein, sequesters host NF-kappa-B in inclusion bodies (IBs) thereby inhibiting this host defense pathway. May also act as a modulator of the innate immune response by sequestration of host IFIH1/MDA5 and MAVS into IBs. 1. Tawar, RG. et al. (2009) Science (New York, N.Y.) 326, 1279-83. PMID: 19965480 2. Bakker, SE. et al. (2013) The Journal of general virology 94, 1734-1738. PMID: 23677789 3. Alvarez Paggi, D. et al. (2019) Archives of biochemistry and biophysics 671, 77-86. PMID: 31229488 4. Galloux, M. et al. (2012) Journal of virology 86, 8375-87. PMID: 22623798 5. Fearns, R. et al. (1997) Virology 236, 188-201. PMID: 9299631 6. Jobe, F. et al. (2020) Journal of virology 94. PMID: 32878896 7. Lifland, AW. et al. (2012) Journal of virology 86, 8245-58. PMID: 22623778
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant HRSV Nucleoprotein, N-His

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Datasheet

Recombinant HRSV Nucleoprotein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Stock Solution

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Working Solution

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Recombinant HRSV Nucleoprotein, N-His (VK088012)

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