Human ABCB10 Recombinant Protein (N-His) (HV570012)

Name: Human ABCB10 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV570012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV570012
Description	Recombinant Human ABCB10 Protein, N-His (HV570012) expressed in E. coli, spanning Leu492-Ala738. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9NRK6
Protein length	Leu492-Ala738
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	29.14 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	M-ABC2, ABC transporter 10 protein, Mitochondrial ATP-binding cassette 2, ATP-binding cassette sub-family B member 10, mitochondrial, ABCB10, ATP-binding cassette transporter 10
Background	ATP-binding cassette sub-family B member 10, mitochondrial (ABCB10) is a ~79 kDa protein. ATP-dependent transporter located in the mitochondrial inner membrane that catalyzes the export of biliverdin from the mitochondrial matrix, and plays a crucial role in hemoglobin synthesis and antioxidative stress. Participates in the early step of the heme biosynthetic process during insertion of iron into protoporphyrin IX (PPIX). Involved in the stabilization of the iron transporter mitoferrin-1/SLC25A37. In addition may be involved in mitochondrial unfolded protein response (UPRmt) signaling pathway, although ABCB10 probably does not participate in peptide export from mitochondria. 1. Tang, L. et al. (2012) British journal of haematology 157, 151-4. PMID: 22085049 2. Yano, M. (2017) Biochemical and biophysical research communications 486, 465-469. PMID: 28315685 3. Seguin, A. et al. (2017) The Journal of biological chemistry 292, 16284-16299. PMID: 28808058 4. Shum, M. et al. (2021) Science translational medicine 13. PMID: 34011630 5. Cao, S. et al. (2023) Nature communications 14, 2030. PMID: 37041204
Note	For research use only.

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SDS-PAGE for Recombinant Human ABCB10 Protein, N-His

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Datasheet

Human ABCB10 Recombinant Protein (N-His).pdf

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Human ABCB10 Recombinant Protein (N-His) (HV570012)

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