ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
28.84 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
MOAT-B, MOATB, Multi-specific organic anion transporter B, ATP-binding cassette sub-family C member 4, Multidrug resistance-associated protein 4, MRP4, ABCC4, MRP/cMOAT-related ABC transporter
Background
ATP-binding cassette sub-family C member 4 (ABCC4) is a ~149 kDa protein. ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins. Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4. Mediates the cotransport of bile acids with reduced glutathione (GSH).
1. van Aubel, RAMH. et al. (2002) Journal of the American Society of Nephrology : JASN 13, 595-603. PMID: 11856762
2. Zelcer, N. et al. (2003) The Biochemical journal 371, 361-7. PMID: 12523936
3. Sauna, ZE. et al. (2004) The Journal of biological chemistry 279, 48855-64. PMID: 15364914
4. Zhu, Y. et al. (2024) FEBS letters 598, 1967-1980. PMID: 38886124
5. Xie, Z. et al. (2025) Cell reports 44, 115466. PMID: 40138312
6. Reid, G. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 9244-9. PMID: 12835412
7. Rius, M. et al. (2003) Hepatology (Baltimore, Md.) 38, 374-84. PMID: 12883481
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human ABCC4 Protein, N-His
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