Human ABCD4 Recombinant Protein (N-His) (HT035012)

Name: Human ABCD4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT035012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT035012
Description	Recombinant Human ABCD4 Protein, N-His (HT035012) expressed in E. coli, spanning Leu356-Glu606. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O14678
Protein length	Leu356-Glu606
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ATP-binding cassette sub-family D member 4, ABCD4, PXMP1-L, PMP70-related protein, Peroxisomal membrane protein 1-like, P70R, PMP69, Peroxisomal membrane protein 69, PXMP1L, Lysosomal cobalamin transporter ABCD4
Background	Lysosomal cobalamin transporter ABCD4 is a ~68 kDa protein. Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner. Targeted by LMBRD1 lysosomal chaperone from the endoplasmic reticulum to the lysosomal membrane. Then forms a complex with lysosomal chaperone LMBRD1 and cytosolic MMACHC to transport cobalamin across the lysosomal membrane. 1. Coelho, D. et al. (2012) Nature genetics 44, 1152-5. PMID: 22922874 2. Fettelschoss, V. et al. (2017) The Journal of biological chemistry 292, 11980-11991. PMID: 28572511 3. Xu, D. et al. (2019) Cell research 29, 1039-1041. PMID: 31467407 4. Kitai, K. et al. (2021) The Journal of biological chemistry 296, 100654. PMID: 33845046 5. Kawaguchi, K. et al. (2016) Scientific reports 6, 30183. PMID: 27456980 6. Deme, JC. et al. (2014) Molecular membrane biology 31, 250-61. PMID: 25535791
Note	For research use only.

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SDS-PAGE for Recombinant Human ABCD4 Protein, N-His

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Datasheet

Human ABCD4 Recombinant Protein (N-His).pdf

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Human ABCD4 Recombinant Protein (N-His) (HT035012)

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