ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
48.26 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
ACAD9, Acyl-CoA dehydrogenase family member 9, ACAD-9, Complex I assembly factor ACAD9, mitochondrial
Background
Complex I assembly factor ACAD9, mitochondrial is a ~68 kDa protein. Together with NDUFAF1 and ECSIT, forms part of the mitochondrial complex I (MCIA),which is required for the biogenesis of respiratory Complex I (CI) and is therefore crucial for the activation of the oxidative phosphorylation system. ECSIT binding triggers a large conformational change, switching ACAD9 from a fatty acid oxidation (FAO) enzyme to a CI assembly factor. The function in CI assembly is independent of the FAO activity of the protein. As FAO enzyme, it catalyzes the first step in FAO, which consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA). Its preferred substrates are both saturated and unsaturated long-chain acyl-CoA substrates, with optimum activity toward the latter.
1. Nouws, J. et al. (2010) Cell metabolism 12, 283-94. PMID: 20816094
2. Nouws, J. et al. (2014) Human molecular genetics 23, 1311-9. PMID: 24158852
3. Formosa, LE. et al. (2020) Cell reports 31, 107541. PMID: 32320651
4. McGregor, L. et al. (2023) Nature communications 14, 8248. PMID: 38086790
5. Zhang, J. et al. (2002) Biochemical and biophysical research communications 297, 1033-42. PMID: 12359260
6. Ensenauer, R. et al. (2005) The Journal of biological chemistry 280, 32309-16. PMID: 16020546
7. He, M. et al. (2007) American journal of human genetics 81, 87-103. PMID: 17564966
8. He, M. et al. (2011) Molecular genetics and metabolism 102, 418-29. PMID: 21237683
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human ACAD9 Protein, N-His
中文
English
한국어
日本語
Español
Français
Русский
