Human Adenylate cyclase type 10/ADCY10 Recombinant Protein (N-His) (HA409012)

Name: Human Adenylate cyclase type 10/ADCY10 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA409012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA409012
Description	Recombinant Human Adenylate cyclase type 10/ADCY10 Protein, N-His (HA409012) expressed in E. coli, spanning Met1-Gln461. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96PN6
Protein length	Met1-Gln461
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	54.45 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Adenylate cyclase type 10; EC:4.6.1.1; AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; hsAC; sAC; Testicular soluble adenylyl cyclase; ADCY10; SAC
Background	Adenylate cyclase type 10 (ADCY10) is a ~187 kDa protein. Catalyzes the formation of the signaling molecule cAMP. May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels. Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. Involved in ciliary beat regulation. 1. Litvin, TN. et al. (2003) The Journal of biological chemistry 278, 15922-6. PMID: 12609998 2. Geng, W. et al. (2005) American journal of physiology. Cell physiology 288, C1305-16. PMID: 15659711 3. Kleinboelting, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 3727-32. PMID: 24567411 4. Saalau-Bethell, SM. et al. (2014) ChemMedChem 9, 823-32. PMID: 24616449 5. Kleinboelting, S. et al. (2014) The FEBS journal 281, 4151-64. PMID: 25040695 6. Schmid, A. et al. (2007) The Journal of general physiology 130, 99-109. PMID: 17591988
Note	For research use only.

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SDS-PAGE for Recombinant Human Adenylate cyclase type 10/ADCY10 Protein, N-His

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Datasheet

Human Adenylate cyclase type 10/ADCY10 Recombinant Protein (N-His).pdf

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Human Adenylate cyclase type 10/ADCY10 Recombinant Protein (N-His) (HA409012)

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