Human ADGRB1 Recombinant Protein (N-GST) (HT218012)

Name: Human ADGRB1 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HT218012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT218012
Description	Recombinant Human ADGRB1 Protein, N-GST (HT218012) expressed in E. coli, spanning Ala31-Pro350. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O14514
Protein length	Ala31-Pro350
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	BAI1, Adhesion G protein-coupled receptor B1, Vstat120, ADGRB1, Vstat40, Brain-specific angiogenesis inhibitor 1
Background	Adhesion G protein-coupled receptor B1 (ADGRB1/BAI1) is a ~173 kDa protein. Phosphatidylserine receptor which enhances the engulfment of apoptotic cells. Also mediates the binding and engulfment of Gram-negative bacteria. Stimulates production of reactive oxygen species by macrophages in response to Gram-negative bacteria, resulting in enhanced microbicidal macrophage activity. In the gastric mucosa, required for recognition and engulfment of apoptotic gastric epithelial cells. Promotes myoblast fusion. 1. Das, S. et al. (2014) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 28, 2214-24. PMID: 24509909 2. Billings, EA. et al. (2016) Science signaling 9, ra14. PMID: 26838550 3. Stephenson, JR. et al. (2013) The Journal of biological chemistry 288, 22248-56. PMID: 23782696 4. Duda, DG. et al. (2002) British journal of cancer 86, 490-6. PMID: 11875720
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human ADGRB1 Protein, N-GST

References

HT218012-SDS-PAGE-1.jpg

Datasheet

Human ADGRB1 Recombinant Protein (N-GST).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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Human ADGRB1 Recombinant Protein (N-GST) (HT218012)

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Datasheet

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