Human ADGRF1 Recombinant Protein (N-His) (HD298012)

Name: Human ADGRF1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD298012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD298012
Description	Recombinant Human ADGRF1 Protein, N-His (HD298012) expressed in E. coli, spanning Thr298-Pro575. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q5T601
Protein length	Thr298-Pro575
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	32.95 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ADGRF1, ADGRF1 C-terminal fragment, ADGRF1 N-terminal fragment, Adhesion G-protein coupled receptor F1, Adhesion G-protein coupled receptor F1, C-terminal fragment, Adhesion G-protein coupled receptor F1, N-terminal fragment, G protein-coupled receptor 110, G protein-coupled receptor KPG_012, GPR110, PGR19
Background	Adhesion G-protein coupled receptor F1 is a ~101 kDa protein. Adhesion G-protein coupled receptor (aGPCR) for N-docosahexaenoylethanolamine (synaptamide), an omega-3 fatty acid lipid highly enriched in the brain. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. ADGRF1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. Also able to couple to G(q), G(i) and G(12)/G(13) G proteins; additional evidence is however required to confirm this result in vivo. Involved in the development of neurons and cognitive function. 1. Lee, JW. et al. (2016) Nature communications 7, 13123. PMID: 27759003 2. Huang, BX. et al. (2020) Communications biology 3, 109. PMID: 32144388 3. Qu, X. et al. (2022) Nature 604, 779-785. PMID: 35418679 4. Zhu, X. et al. (2022) Nature communications 13, 5513. PMID: 36127364 5. Jones, DTD. et al. (2023) Nature communications 14, 2490. PMID: 37120430
Note	For research use only

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Datasheet

Human ADGRF1 Recombinant Protein (N-His).pdf

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Human ADGRF1 Recombinant Protein (N-His) (HD298012)

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