Human ALDOB Recombinant Protein (N-His) (HY240012)

Name: Human ALDOB Recombinant Protein (N-His)
Brand: abinScience
SKU: HY240012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY240012
Description	Recombinant Human ALDOB Protein, N-His (HY240012) expressed in E. coli, spanning Thr9-Tyr364. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P05062
Protein length	Thr9-Tyr364
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	40.84 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ALDOB, Liver-type aldolase, Fructose-bisphosphate aldolase B, ALDB
Background	Fructose-bisphosphate aldolase B (ALDOB) is a ~39 kDa protein. Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde. Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check. 1. Santamaria, R. et al. (2000) The Biochemical journal 350 Pt 3, 823-8. PMID: 10970798 2. Sánchez-Gutiérrez, JC. et al. (2002) Journal of medical genetics 39, e56. PMID: 12205126 3. Esposito, G. et al. (2010) Human mutation 31, 1294-303. PMID: 20848650 4. Li, M. et al. (2020) Nature cancer 1, 735-747. PMID: 35122041
Note	For research use only.

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SDS-PAGE for Recombinant Human ALDOB Protein, N-His

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Datasheet

Human ALDOB Recombinant Protein (N-His).pdf

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Human ALDOB Recombinant Protein (N-His) (HY240012)

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