Human ANP32B Recombinant Protein (N-His) (HV051022)

Name: Human ANP32B Recombinant Protein (N-His)
Brand: abinScience
SKU: HV051022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV051022
Description	Recombinant Human ANP32B Protein, N-His (HV051022) expressed in E. coli, spanning Met1-Glu151. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q92688
Protein length	Met1-Glu151
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.40 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Acidic leucine-rich nuclear phosphoprotein 32 family member B; Acidic protein rich in leucines; Putative HLA-DR-associated protein I-2; PHAPI2; Silver-stainable protein SSP29; ANP32B; APRIL; PHAPI2
Background	Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) is a ~28 kDa protein. Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription. Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis. Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes. Also plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1. 1. Munemasa, Y. et al. (2008) Molecular and cellular biology 28, 1171-81. PMID: 18039846 2. Shen, SM. et al. (2010) Carcinogenesis 31, 419-26. PMID: 20015864 3. Tochio, N. et al. (2010) Journal of molecular biology 401, 97-114. PMID: 20538007 4. Fries, B. et al. (2007) The Journal of biological chemistry 282, 4504-4515. PMID: 17178712
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human ANP32B Protein, N-His

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Datasheet

Human ANP32B Recombinant Protein (N-His).pdf

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Formula

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Human ANP32B Recombinant Protein (N-His) (HV051022)

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