ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
31.56 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
DNA repair nuclease/redox regulator APEX1 is a ~35 kDa protein. Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Also incises at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules.
1. Wei, SJ. et al. (2000) Cancer research 60, 6688-95. PMID: 11118054
2. Hsieh, MM. et al. (2001) Nucleic acids research 29, 3116-22. PMID: 11452037
3. Parsons, JL. et al. (2005) Nucleic acids research 33, 2204-9. PMID: 15831793
4. Berquist, BR. et al. (2008) Journal of molecular biology 379, 17-27. PMID: 18439621
5. Georgiadis, MM. et al. (2008) Mutation research 643, 54-63. PMID: 18579163
6. Kim, WC. et al. (2011) Journal of molecular biology 411, 960-71. PMID: 21762700
7. Schermerhorn, KM. et al. (2013) Biochemistry 52, 7669-77. PMID: 24079850
8. Walker, LJ. et al. (1993) Molecular and cellular biology 13, 5370-6. PMID: 8355688
9. Hirota, K. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 3633-8. PMID: 9108029
10. Ramana, CV. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 5061-6. PMID: 9560228
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human APEX1 Protein, N-His
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