Human ARHGEF15 Recombinant Protein (N-His) (HB586012)

Name: Human ARHGEF15 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB586012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB586012
Description	Recombinant Human ARHGEF15 Protein, N-His (HB586012) expressed in E. coli, spanning Arg619-Thr741. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O94989
Protein length	Arg619-Thr741
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.24 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Rho guanine nucleotide exchange factor 15; Ephexin-5; E5; Vsm-RhoGEF; ARHGEF15; KIAA0915
Background	Rho guanine nucleotide exchange factor 15 is a ~91 kDa protein. Guanine nucleotide exchange factor (GEF) that activates RhoA, playing a role in the regulation of actin cytoskeleton organization. Is involved in the regulation of vascular smooth muscle contractility. Additionally, it negatively regulates excitatory synapse development by suppressing the synapse-promoting activity of EPHB2. Does not activate RAC1 or CDC42. 1. Ding, X. et al. (2023) Acta neuropathologica 145, 681-705. PMID: 36929019 2. Ogita, H. et al. (2003) Circulation research 93, 23-31. PMID: 12775584
Note	For research use only

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SDS-PAGE for Recombinant Human ARHGEF15 Protein, N-His

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Datasheet

Human ARHGEF15 Recombinant Protein (N-His).pdf

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Formula

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Human ARHGEF15 Recombinant Protein (N-His) (HB586012)

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