Human ATF7IP Recombinant Protein (N-His) (HB362012)

Name: Human ATF7IP Recombinant Protein (N-His)
Brand: abinScience
SKU: HB362012
Price: 211 USD
Availability: InStock

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Catalog No.	HB362012
Description	Recombinant Human ATF7IP Protein, N-His (HB362012) expressed in E. coli, spanning Gln1149-Ser1270. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6VMQ6
Protein length	Gln1149-Ser1270
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	15.79 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Activating transcription factor 7-interacting protein 1; ATF-interacting protein; ATF-IP; ATF7-interacting protein; ATFa-associated modulator; hAM; MBD1-containing chromatin-associated factor 1; P621; ATF7IP; MCAF; MCAF1
Background	Activating transcription factor 7-interacting protein 1 is a ~136 kDa protein. Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Required for HUSH-mediated heterochromatin formation and gene silencing. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Stabilizes SETDB1, is required to stimulate histone methyltransferase activity of SETDB1 and facilitates the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). 1. Timms, RT. et al. (2016) Cell reports 17, 653-659. PMID: 27732843 2. Fujita, N. et al. (2003) Molecular and cellular biology 23, 2834-43. PMID: 12665582 3. Wang, H. et al. (2003) Molecular cell 12, 475-87. PMID: 14536086 4. Liu, L. et al. (2009) The Journal of biological chemistry 284, 5165-74. PMID: 19106100
Note	For research use only

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SDS-PAGE for Recombinant Human ATF7IP Protein, N-His

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Datasheet

Human ATF7IP Recombinant Protein (N-His).pdf

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Human ATF7IP Recombinant Protein (N-His) (HB362012)

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