Human ATP11A Recombinant Protein (N-His) (HB310012)

Name: Human ATP11A Recombinant Protein (N-His)
Brand: abinScience
SKU: HB310012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB310012
Description	Recombinant Human ATP11A Protein, N-His (HB310012) expressed in E. coli, spanning Asp387-Lys860. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P98196
Protein length	Asp387-Lys860
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	55.07 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Phospholipid-transporting ATPase IH; 7.6.2.1; ATPase IS; ATPase class VI type 11A; P4-ATPase flippase complex alpha subunit ATP11A; ATP11A; ATPIH; ATPIS; KIAA1021
Background	Phospholipid-transporting ATPase IH is a ~129 kDa protein. Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of the plasma membrane. Does not show flippase activity toward phosphatidylcholine (PC). Contributes to the maintenance of membrane lipid asymmetry with a specific role in morphogenesis of muscle cells. In myoblasts, mediates PS enrichment at the inner leaflet of plasma membrane, triggering PIEZO1-dependent Ca2+ influx and Rho GTPases signal transduction, subsequently leading to the assembly of cortical actomyosin fibers and myotube formation. May be involved in the uptake of farnesyltransferase inhibitor drugs, such as lonafarnib. 1. Takatsu, H. et al. (2014) The Journal of biological chemistry 289, 33543-56. PMID: 25315773 2. Naito, T. et al. (2015) The Journal of biological chemistry 290, 15004-17. PMID: 25947375 3. Segawa, K. et al. (2016) The Journal of biological chemistry 291, 762-72. PMID: 26567335 4. Tsuchiya, M. et al. (2018) Nature communications 9, 2049. PMID: 29799007 5. Wang, J. et al. (2018) Scientific reports 8, 10795. PMID: 30018401 6. Chepurwar, S. et al. (2023) Human molecular genetics 32, 1083-1089. PMID: 36300302 7. Segawa, K. et al. (2021) The Journal of clinical investigation 131. PMID: 34403372
Note	For research use only

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SDS-PAGE for Recombinant Human ATP11A Protein, N-His

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Datasheet

Human ATP11A Recombinant Protein (N-His).pdf

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Formula

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Human ATP11A Recombinant Protein (N-His) (HB310012)

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