

| Catalog No. | HW654022 |
|---|---|
| Description |
Recombinant Human ATP6V1E1 Protein, N-His (HW654022) expressed in E. coli, spanning Met1-Asp226. Purity: >90% by SDS-PAGE.
Highlights
|
| Expression system | E. coli |
| Accession | P36543 |
| Protein length | Met1-Asp226 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 28.31 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | ATP6E, ATP6E2, ATP6V1E1, V-ATPase 31 kDa subunit, V-ATPase subunit E 1, V-type proton ATPase subunit E 1, Vacuolar proton pump subunit E 1, p31 |
| Background | V-type proton ATPase subunit E 1 is a ~26 kDa protein. Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. 1. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091 2. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002 |
| Note | For research use only |
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