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Human AWAT2 Recombinant Protein (N-His) (HD881012)

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Overview
Catalog No.HD881012
Description
Recombinant Human AWAT2 Protein, N-His (HD881012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionQ6E213
Protein lengthGly66-Ile333
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names11-cis-RE-synthase, 11-cis-specific retinyl-ester synthase, ARAT, AWAT2, Acyl-CoA retinol O-fatty-acyltransferase, Acyl-CoA wax alcohol acyltransferase 2, DC4, DGAT2L4, Diacylglycerol O-acyltransferase 2-like protein 4, Diacylglycerol O-acyltransferase candidate 4, EC:2.3.1.20, EC:2.3.1.75, EC:2.3.1.76, Long-chain-alcohol O-fatty-acyltransferase 2, MFAT, Multifunctional O-acyltransferase, Retinol O-fatty-acyltransferase, WS, Wax synthase, hDC4, hWS
Background

Acyl-CoA wax alcohol acyltransferase 2 is a ~38 kDa protein. Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters. Shows a preference for medium chain acyl-CoAs from C12 to C16 in length and fatty alcohols shorter than C20, as the acyl donors and acceptors, respectively. Also possesses acyl-CoA retinol acyltransferase (ARAT) activity that preferentially esterifies 11-cis-retinol, a chromophore precursor of bleached opsin pigments in cone cells. Shows higher catalytic efficiency toward 11-cis-retinol versus 9-cis-retinol, 13-cis-retinol, and all-trans-retinol substrates.

1. Cheng, JB. et al. (2004) The Journal of biological chemistry 279, 37798-807. PMID: 15220349
2. Turkish, AR. et al. (2005) The Journal of biological chemistry 280, 14755-64. PMID: 15671038
3. Yen, CL. et al. (2005) Journal of lipid research 46, 2388-97. PMID: 16106050
4. Ma, Z. et al. (2017) Journal of lipid research 58, 1091-1099. PMID: 28420705
5. Kaylor, JJ. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 7302-7. PMID: 24799687
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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