Recombinant Human B4GALT1 Protein, C-Fc (HC014011) expressed in Mammalian Cells, spanning Arg45-Ser398.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian cells
Accession
P15291
Protein length
Arg45-Ser398
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Predicted molecular weight
65.70 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Beta-1,4-galactosyltransferase 1 is a ~43 kDa protein. Galactosyltransferase acting in the Golgi stacks. Catalyzes the transfer of galactose (Gal) from UDP-alpha-D-galactose in beta(1->4) linkage to the non-reducing terminal N-acetylglucosamine (GlcNAc) moieties of glycolipids and complex-type N-linked glycans. Adds one Gal residue to both GlcNAc beta(1->2)-linked to the alpha(1->3) and alpha(1->6) mannose antennae of complex-type N-glycans, enabling the formation of mono- and di-galactosylated glycoforms. Galactosylates complex-type N-glycans attached on the fragment crystallizable (Fc) of immunoglobulin-gamma isotypes (IgGs), a prerequisite for antibody glycan sialylation and related anti-inflammatory effector functions. Can also transfer a Gal residue to free GlcNAc to form N-acetyllactosamine.
1. Ramasamy, V. et al. (2005) Journal of molecular biology 353, 53-67. PMID: 16157350
2. Dekkers, G. et al. (2016) Scientific reports 6, 36964. PMID: 27872474
3. Benedetti, E. et al. (2017) Nature communications 8, 1483. PMID: 29133956
4. Jaroentomeechai, T. et al. (2022) Nature communications 13, 6325. PMID: 36280670
5. Ma, W. et al. (2023) Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10, e2303832. PMID: 37632720
6. Makrydaki, E. et al. (2024) Nature chemical biology 20, 732-741. PMID: 38321209
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant Human B4GALT1 Protein, C-Fc
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