ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
45.71 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
BRE, BRCA1/BRCA2-containing complex subunit 45, BRCA1-A complex subunit BRE, BRISC and BRCA1-A complex member 2, Brain and reproductive organ-expressed protein, BABAM2, BRCC45
Background
BRISC and BRCA1-A complex member 2 (BABAM2) is a ~43 kDa protein. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Within the BRISC complex, acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity.
1. Sobhian, B. et al. (2007) Science (New York, N.Y.) 316, 1198-202. PMID: 17525341
2. Shao, G. et al. (2009) Genes & development 23, 740-54. PMID: 19261746
3. Feng, L. et al. (2009) Genes & development 23, 719-28. PMID: 19261748
4. Wang, B. et al. (2009) Genes & development 23, 729-39. PMID: 19261749
5. Hu, X. et al. (2011) The Journal of biological chemistry 286, 11734-45. PMID: 21282113
6. Cooper, EM. et al. (2009) The EMBO journal 28, 621-31. PMID: 19214193
7. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985
8. Zhang, J. et al. (2014) Nature communications 5, 5059. PMID: 25283148
9. Yan, K. et al. (2015) The Journal of cell biology 210, 209-24. PMID: 26195665
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human BABAM2 Protein, N-His
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