ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
20.85 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Aspartyl protease 1, Membrane-associated aspartic protease 1, Beta-secretase 2, ASP21, BACE2, Asp 1, ALP56, Beta-site APP cleaving enzyme 2, Aspartic-like protease 56 kDa, Beta-site amyloid precursor protein cleaving enzyme 2, Down region aspartic protease, Theta-secretase, Memapsin-1, AEPLC, ASP1, DRAP
Background
Beta-secretase 2 (BACE2) is a ~56 kDa protein. Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Involved in the proteolytic shedding of PMEL at early stages of melanosome biogenesis. Cleaves PMEL within the M-beta fragment to release the amyloidogenic PMEL luminal fragment containing M-alpha and a small portion of M-beta N-terminus.
1. Yan, R. et al. (1999) Nature 402, 533-7. PMID: 10591213
2. Hussain, I. et al. (2000) Molecular and cellular neurosciences 16, 609-19. PMID: 11083922
3. Yan, R. et al. (2001) The Journal of biological chemistry 276, 34019-27. PMID: 11423558
4. Sun, X. et al. (2005) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 19, 739-49. PMID: 15857888
5. Sun, X. et al. (2006) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20, 1369-76. PMID: 16816112
6. Rochin, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10658-63. PMID: 23754390
7. Esterházy, D. et al. (2011) Cell metabolism 14, 365-77. PMID: 21907142
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human BACE2 Protein, N-His
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