ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
45.73 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BAX, BCL2L4
Background
Apoptosis regulator BAX is a ~21 kDa protein. Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
1. Schmitt, E. et al. (2000) Biochemical and biophysical research communications 270, 868-79. PMID: 10772918
2. Tan, KO. et al. (2001) The Journal of biological chemistry 276, 2802-7. PMID: 11060313
3. Zhang, H. et al. (2005) Nature cell biology 7, 909-15. PMID: 16113678
4. Tan, KO. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 14623-8. PMID: 16199525
5. Gavathiotis, E. et al. (2008) Nature 455, 1076-81. PMID: 18948948
6. Czabotar, PE. et al. (2011) The Journal of biological chemistry 286, 7123-31. PMID: 21199865
7. Edlich, F. et al. (2011) Cell 145, 104-16. PMID: 21458670
8. Wei, B. et al. (2015) Journal of virology 89, 3804-18. PMID: 25609812
9. Choi, HS. et al. (2022) International journal of molecular sciences 23. PMID: 36361894
中文
English
한국어
日本語
Español
Français
Русский
