ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
22.83 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
BIRC7, p30-Livin, Melanoma inhibitor of apoptosis protein, LIVIN, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein, Truncated livin, RNF50, KIAP, Livin, RING-type E3 ubiquitin transferase BIRC7, RING finger protein 50, tLivin, MLIAP, ML-IAP
Background
Baculoviral IAP repeat-containing protein 7 (BIRC7) is a ~32 kDa protein. Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine.
1. Kasof, GM. et al. (2001) The Journal of biological chemistry 276, 3238-46. PMID: 11024045
2. Vucic, D. et al. (2000) Current biology : CB 10, 1359-66. PMID: 11084335
3. Lin, JH. et al. (2000) Biochemical and biophysical research communications 279, 820-31. PMID: 11162435
4. Ma, L. et al. (2006) Cell death and differentiation 13, 2079-88. PMID: 16729033
5. Nachmias, B. et al. (2007) Apoptosis : an international journal on programmed cell death 12, 1129-42. PMID: 17294084
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human BIRC7 Protein, N-His-SUMO
中文
English
한국어
日本語
Español
Français
Русский
