Human BLOC1S2 Recombinant Protein (N-His) (HB360012)

Name: Human BLOC1S2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB360012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB360012
Description	Recombinant Human BLOC1S2 Protein, N-His (HB360012) expressed in E. coli, spanning Glu34-Arg142. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6QNY1
Protein length	Glu34-Arg142
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	14.93 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Biogenesis of lysosome-related organelles complex 1 subunit 2; BLOC-1 subunit 2; Centrosome-associated protein; BLOC1S2; BLOS2; CEAP
Background	Biogenesis of lysosome-related organelles complex 1 subunit 2 is a ~15 kDa protein. Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor. 1. Starcevic, M. et al. (2004) The Journal of biological chemistry 279, 28393-401. PMID: 15102850 2. Setty, SR. et al. (2007) Molecular biology of the cell 18, 768-80. PMID: 17182842 3. Pu, J. et al. (2015) Developmental cell 33, 176-88. PMID: 25898167 4. Wang, Z. et al. (2004) Journal of molecular biology 343, 71-82. PMID: 15381421
Note	For research use only

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SDS-PAGE for Recombinant Human BLOC1S2 Protein, N-His

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Datasheet

Human BLOC1S2 Recombinant Protein (N-His).pdf

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Human BLOC1S2 Recombinant Protein (N-His) (HB360012)

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