Human BMP6 Recombinant Protein (N-His) (HB857012)

Name: Human BMP6 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB857012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB857012
Description	Recombinant Human BMP6 Protein, N-His (HB857012) expressed in E. coli, spanning Ser375-His513. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P22004
Protein length	Ser375-His513
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.97 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	VG-1-R, VG-1-related protein, Bone morphogenetic protein 6, VGR-1, BMP-6, VGR, BMP6
Background	Bone morphogenetic protein 6 (BMP6) is a ~57 kDa protein. Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes including cartilage and bone formation. Also plays an important role in the regulation of HAMP/hepcidin expression and iron metabolism by acting as a ligand for hemojuvelin/HJV. Also acts to promote expression of HAMP, potentially via the interaction with its receptor BMPR1A/ALK3. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target. 1. Seeherman, HJ. et al. (2019) Science translational medicine 11. PMID: 31019025 2. Daher, R. et al. (2016) Gastroenterology 150, 672-683.e4. PMID: 26582087 3. Arezes, J. et al. (2018) Blood 132, 1473-1477. PMID: 30097509 4. Wang, CY. et al. (2020) Blood 135, 453-456. PMID: 31800957 5. Saremba, S. et al. (2008) The FEBS journal 275, 172-83. PMID: 18070108 6. Pulkkinen, HH. et al. (2021) Angiogenesis 24, 129-144. PMID: 33021694
Note	For research use only.

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SDS-PAGE for Recombinant Human BMP6 Protein, N-His

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Datasheet

Human BMP6 Recombinant Protein (N-His).pdf

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Human BMP6 Recombinant Protein (N-His) (HB857012)

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