Human BRAT1 Recombinant Protein (N-His) (HD348012)

Name: Human BRAT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD348012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD348012
Description	Recombinant Human BRAT1 Protein, N-His (HD348012) expressed in E. coli, spanning Met1-Gly291. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6PJG6
Protein length	Met1-Gly291
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	BAAT1, BRAT1, BRCA1-associated ATM activator 1, BRCA1-associated protein required for ATM activation protein 1, C7orf27, Integrator complex assembly factor BRAT1
Background	Integrator complex assembly factor BRAT1 is a ~88 kDa protein. Component of a multiprotein complex required for the assembly of the RNA endonuclease module of the integrator complex. Associates with INTS9 and INTS11 in the cytoplasm and blocks the active site of INTS11 to inhibit the endonuclease activity of INTS11 before formation of the full integrator complex. Following dissociation of WDR73 of the complex, BRAT1 facilitates the nuclear import of the INTS9-INTS11 heterodimer. In the nucleus, INTS4 is integrated to the INTS9-INTS11 heterodimer and BRAT1 is released from the mature RNA endonuclease module by inositol hexakisphosphate (InsP6). BRAT1 is also involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress. 1. Sabath, K. et al. (2024) Molecular cell 84, 2882-2899.e10. PMID: 39032489 2. Lin, MH. et al. (2024) Molecular cell 84, 2900-2917.e10. PMID: 39032490 3. Aglipay, JA. et al. (2006) The Journal of biological chemistry 281, 9710-8. PMID: 16452482 4. So, EY. et al. (2011) Experimental and therapeutic medicine 2, 443-447. PMID: 22977523 5. So, EY. et al. (2014) BMC cancer 14, 548. PMID: 25070371 6. So, EY. et al. (2013) Journal of cancer biology & research 1. PMID: 25657994
Note	For research use only

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Human BRAT1 Recombinant Protein (N-His).pdf

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Human BRAT1 Recombinant Protein (N-His) (HD348012)

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