Human BRCA1 Recombinant Protein (N-His) (HW337012)

Name: Human BRCA1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW337012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW337012
Description	Recombinant Human BRCA1 Protein, N-His (HW337012) expressed in E. coli, spanning Gln19-Val83. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P38398
Protein length	Gln19-Val83
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	9.79 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	BRCA1, Breast cancer type 1 susceptibility protein, RING-type E3 ubiquitin transferase BRCA1, RING finger protein 53, RNF53
Background	Breast cancer type 1 susceptibility protein (BRCA1) is a ~207 kDa protein. E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. 1. Lorick, KL. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 11364-9. PMID: 10500182 2. Vandenberg, CJ. et al. (2003) Molecular cell 12, 247-54. PMID: 12887909 3. Wu-Baer, F. et al. (2003) The Journal of biological chemistry 278, 34743-6. PMID: 12890688 4. Morris, JR. et al. (2004) Human molecular genetics 13, 807-17. PMID: 14976165 5. Yu, X. et al. (2006) Genes & development 20, 1721-6. PMID: 16818604 6. Wang, B. et al. (2007) Science (New York, N.Y.) 316, 1194-8. PMID: 17525340 7. Feng, L. et al. (2009) Genes & development 23, 719-28. PMID: 19261748 8. Wu-Baer, F. et al. (2010) Molecular and cellular biology 30, 2787-98. PMID: 20351172
Note	For research use only.

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SDS-PAGE for Recombinant Human BRCA1 Protein, N-His

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HW337012-SDS-PAGE-1.jpg

Datasheet

Human BRCA1 Recombinant Protein (N-His).pdf

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Human BRCA1 Recombinant Protein (N-His) (HW337012)

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