Human CCKBR/CCK2-R Recombinant Protein (N-His-KSI) (HB288032)

Name: Human CCKBR/CCK2-R Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HB288032
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB288032
Description	Recombinant Human CCKBR/CCK2-R Protein, N-His-KSI (HB288032) expressed in E. coli, spanning Arg243-Arg333. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P32239
Protein length	Arg243-Arg333
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.46 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK-BR, Cholecystokinin-2 receptor, CCK2-R, CCKBR
Background	Gastrin/cholecystokinin type B receptor (CCKBR/CCK2-R) is a ~48 kDa protein. Receptor for the peptide hormones gastrin and cholecystokinin (CCK). Expressed throughout the central nervous system, where it modulates processes such as anxiety, analgesia, arousal and neuroleptic activity. Couples to both GNAI1 and GNAQ signaling pathways, but not to GNAS. Upon gastrin activation, reduces glucose absorption in intestinal epithelial cells by downregulating SGLT1 and GLUT2 expression through suppression of the PI3K/Akt/eIF4B pathway. In the kidney, decreases SGLT2 expression under high-glucose conditions via ERK/NF-kappa-B signaling. 1. Zhang, X. et al. (2021) Nature chemical biology 17, 1230-1237. PMID: 34556863
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CCKBR/CCK2-R Protein, N-His-KSI

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Datasheet

Human CCKBR/CCK2-R Recombinant Protein (N-His-KSI).pdf

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Human CCKBR/CCK2-R Recombinant Protein (N-His-KSI) (HB288032)

Overview

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References

Datasheet

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