Human CCNF Recombinant Protein (N-His) (HW835012)

Name: Human CCNF Recombinant Protein (N-His)
Brand: abinScience
SKU: HW835012
Price: 211 USD
Availability: InStock

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Catalog No.	HW835012
Description	Recombinant Human CCNF Protein, N-His (HW835012) expressed in E. coli, spanning Gly98-Gly541. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P41002
Protein length	Gly98-Gly541
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	52.54 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	FBXO1, F-box only protein 1, Cyclin-F, CCNF, FBX1
Background	Cyclin-F (CCNF) is a ~87 kDa protein. Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The SCF(CCNF) E3 ubiquitin-protein ligase complex is an integral component of the ubiquitin proteasome system (UPS) and links proteasome degradation to the cell cycle. Mediates the substrate recognition and the proteasomal degradation of various target proteins involved in the regulation of cell cycle progression and in the maintenance of genome stability. Mediates the ubiquitination and proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. In G2, mediates the ubiquitination and subsequent degradation of ribonucleotide reductase RRM2, thereby maintaining a balanced pool of dNTPs and genome integrity. 1. D'Angiolella, V. et al. (2010) Nature 466, 138-42. PMID: 20596027 2. D'Angiolella, V. et al. (2012) Cell 149, 1023-34. PMID: 22632967 3. Walter, D. et al. (2016) Nature communications 7, 10530. PMID: 26818844 4. Williams, KL. et al. (2016) Nature communications 7, 11253. PMID: 27080313 5. Choudhury, R. et al. (2016) Cell reports 16, 3359-3372. PMID: 27653696 6. Lee, A. et al. (2018) Cellular and molecular life sciences : CMLS 75, 335-354. PMID: 28852778 7. Bai, C. et al. (1996) Cell 86, 263-74. PMID: 8706131
Note	For research use only.

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SDS-PAGE for Recombinant Human CCNF Protein, N-His

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Human CCNF Recombinant Protein (N-His).pdf

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Human CCNF Recombinant Protein (N-His) (HW835012)

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