Human CCNT1 Recombinant Protein (N-His) (HC948012)

Name: Human CCNT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC948012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC948012
Description	Recombinant Human CCNT1 Protein, N-His (HC948012) expressed in E. coli, spanning Met1-Ser290. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60563
Protein length	Met1-Ser290
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	35.52 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CCNT1, CycT1, Cyclin-T, Cyclin-T1
Background	Cyclin-T1 is a ~80 kDa protein. Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II. 1. Jang, MK. et al. (2005) Molecular cell 19, 523-34. PMID: 16109376 2. Yang, Z. et al. (2005) Molecular cell 19, 535-45. PMID: 16109377 3. Ghosh, K. et al. (2018) Cell reports 24, 2141-2154.e6. PMID: 30134174 4. Fu, H. et al. (2022) Nature cell biology 24, 513-525. PMID: 35393539 5. Lu, H. et al. (2018) Nature 558, 318-323. PMID: 29849146
Note	For research use only

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Datasheet

Human CCNT1 Recombinant Protein (N-His).pdf

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Human CCNT1 Recombinant Protein (N-His) (HC948012)

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