ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
39.62 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
LGP-96, CD107b, LAMP2, CD107 antigen-like family member B, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2, LAMP-2
Background
Lysosome-associated membrane glycoprotein 2 (CD107b/LAMP2) is a ~44 kDa protein. Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH, GPX4, NLRP3 and MLLT11, and targeting them for lysosomal degradation. In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2.
1. Cuervo, AM. et al. (2000) Journal of cell science 113 Pt 24, 4441-50. PMID: 11082038
2. Bandyopadhyay, U. et al. (2008) Molecular and cellular biology 28, 5747-63. PMID: 18644871
3. Li, P. et al. (2014) Experimental cell research 327, 48-56. PMID: 24880125
4. Hubert, V. et al. (2016) Biology open 5, 1516-1529. PMID: 27628032
5. Wang, L. et al. (2023) Molecular cell 83, 281-297.e10. PMID: 36586411
6. Zhang, J. et al. (2023) Molecular cell 83, 2524-2539.e7. PMID: 37390818
7. Cuervo, AM. et al. (1996) Science (New York, N.Y.) 273, 501-3. PMID: 8662539
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD107b/LAMP2 Protein, N-His
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