Human CD124/IL4R Recombinant Protein (C-His) (HB732011)

Name: Human CD124/IL4R Recombinant Protein (C-His)
Brand: abinScience
SKU: HB732011
Price: 298 USD
Availability: InStock

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Spec:

50ug
100ug
1mg

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB732011

Description

Recombinant Human CD124/IL4R Protein, C-His (HB732011) expressed in Mammalian Cells, spanning Met1-His232. Purity: >90% by SDS-PAGE.

Highlights

●His-Tagged — C-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.

Expression system

Mammalian Cells

Accession

P24394

Protein length

Met1-His232

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Species

Homo sapiens (Human)

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Predicted molecular weight

27.26 kDa

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

IL-4RA, IL-4-binding protein, IL-4 receptor subunit alpha, Soluble IL-4R-alpha, IL4-BP, IL4R, IL-4R subunit alpha, IL-4R-alpha, CD124, Interleukin-4 receptor subunit alpha, IL4RA, Soluble IL-4 receptor subunit alpha, sIL4Ralpha/prot

Background

Interleukin-4 receptor subunit alpha (CD124/IL4R) is a ~89 kDa protein. Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. CD124 is the therapeutic target of dupilumab (Dupixent).

1. Andrews, AL. et al. (2006) The Journal of allergy and clinical immunology 118, 858-65. PMID: 17030238

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD124/IL4R Protein, C-His
Bioactivity

Detected by Research Grade Rademikibart (Catalog No: HB732136) in indirect ELISA
Bioactivity

Detected by Stapokibart (Catalog No: HB732146) in indirect ELISA.
Bioactivity

Detected by MEDI2045 (Catalog No: HB732106) in indirect ELISA.