Human CD40 Ligand/TNFSF5 Recombinant Protein (C-Fc) (HB199021)

Name: Human CD40 Ligand/TNFSF5 Recombinant Protein (C-Fc)
Brand: abinScience
SKU: HB199021
Price: 298 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB199021
Description	Recombinant Human CD154/CD40LG/TNFSF5 Protein, C-Fc (HB199021) expressed in Mammalian Cells, spanning Met113-Leu261. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	P29965
Protein length	Met113-Leu261
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	44.02 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CD154, CD40 ligand, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40-L, CD40L, CD40LG, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5, sCD40L
Background	CD40 ligand is a ~29 kDa protein. Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. 1. Graf, D. et al. (1992) European journal of immunology 22, 3191-4. PMID: 1280226 2. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973 3. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933 4. Mikolajczak, SA. et al. (2004) The Journal of experimental medicine 199, 1025-31. PMID: 15067037
Note	For research use only

Images

Bioactivity

Immobilized HB782011 Human CD40/TNFRSF5 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HB199021 Human CD154/CD40LG/TNFSF5 Recombinant Protein (C-Fc), the EC 50 is 98.6 ng/mL.
Bioactivity

Immobilized HB782021 Human CD40/TNFRSF5 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB199021 Human CD154/CD40LG/TNFSF5 Recombinant Protein (C-Fc), the EC 50 is 2.22 μg/mL.