Human CD158d/KIR2DL4 Recombinant Protein (C-His) (HV220021)

Name: Human CD158d/KIR2DL4 Recombinant Protein (C-His)
Brand: abinScience
SKU: HV220021
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	HV220021
Description	Recombinant Human CD158d/KIR2DL4 Protein, C-His (HV220021) expressed in Mammalian Cells, spanning Met1-His242. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	Q99706
Protein length	Met1-His242
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	27.52 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	KIR2DL4, KIR-103AS, KIR103AS, CD158d, CD158 antigen-like family member D, CD158D, MHC class I NK cell receptor KIR103AS, Killer cell immunoglobulin-like receptor 2DL4, G9P, Killer cell inhibitory receptor 103AS
Background	Killer cell immunoglobulin-like receptor 2DL4 (CD158d/KIR2DL4) is a ~41 kDa protein. Receptor for non-classical major histocompatibility class Ib HLA-G molecules. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M). In decidual NK cells, binds peptide-bound HLA-G-B2M complex and triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy. May play a role in balancing tolerance and antiviral-immunity at maternal-fetal interface by keeping in check the effector functions of NK, CD8+ T cells and B cells. Upon interaction with peptide-bound HLA-G-B2M, initiates signaling from the endosomal compartment leading to downstream activation of PRKDC-XRCC5 and AKT1, and ultimately triggering NF-kappa-B-dependent pro-inflammatory response. 1. Rajagopalan, S. et al. (2006) PLoS biology 4, e9. PMID: 16366734 2. Rajagopalan, S. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 20596-601. PMID: 23184984 3. Fu, B. et al. (2017) Immunity 47, 1100-1113.e6. PMID: 29262349 4. Rajagopalan, S. et al. (1999) The Journal of experimental medicine 189, 1093-100. PMID: 10190900 5. Rajagopalan, S. et al. (2010) Science signaling 3, ra14. PMID: 20179272
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CD158d/KIR2DL4 Protein, C-His

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Datasheet

Human CD158d/KIR2DL4 Recombinant Protein (C-His).pdf

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Formula

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Human CD158d/KIR2DL4 Recombinant Protein (C-His) (HV220021)

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