Human CD289/TLR9 Recombinant Protein (N-His) (HV425012)

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Catalog No.	HV425012
Description	Recombinant Human CD289/TLR9 Protein, N-His (HV425012) expressed in E. coli, spanning Asn64-Glu189. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9NR96
Protein length	Asn64-Glu189
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.4 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CD289, TLR9, Toll-like receptor 9
Background	Toll-like receptor 9 (CD289/TLR9) is a ~115 kDa protein. Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also acts via ADCY7, leading to cyclic di-AMP (c-di-AMP) synthesis and activation of the NLRP3 inflammasome. 1. Latz, E. et al. (2004) Nature immunology 5, 190-8. PMID: 14716310 2. Takeshita, F. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 3555-8. PMID: 11564765 3. Doyle, SL. et al. (2007) The Journal of biological chemistry 282, 36953-60. PMID: 17932028 4. Li, FJ. et al. (2013) European journal of immunology 43, 2980-92. PMID: 23857366
Note	For research use only.

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SDS-PAGE for Recombinant Human CD289/TLR9 Protein, N-His

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HV425012-SDS-PAGE-1.jpg

Datasheet

Human CD289/TLR9 Recombinant Protein (N-His).pdf

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Human CD289/TLR9 Recombinant Protein (N-His) (HV425012)

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