Home > Products > Recombinant Proteins > Immune Checkpoint Proteins

Human CD304/NRP1 Recombinant Protein (C-Fc) (HT070011)

Datasheet Share product Copy info

Human CD304/NRP1 Recombinant Protein (C-Fc)

Human CD304/NRP1 Recombinant Protein (C-Fc)

Price(USD): $

Spec:

50ug
100ug
1mg

Number:

Contact us

Overview
Images
References
Datasheet

Overview

Catalog No.	HT070011
Description	Recombinant Human CD304/NRP1 Protein, C-Fc (HT070011) expressed in Mammalian Cells, spanning Met1-Lys644. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	O14786
Protein length	Met1-Lys644
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NRP1, Neuropilin-1, VEGF165R, NRP, CD304, Vascular endothelial cell growth factor 165 receptor, SARS-like Coronaviruses
Background	Neuropilin-1 (CD304/NRP1) is a ~103 kDa protein. Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. 1. Gagnon, ML. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 2573-8. PMID: 10688880 2. He, Z. et al. (1997) Cell 90, 739-51. PMID: 9288753 3. Soker, S. et al. (1998) Cell 92, 735-45. PMID: 9529250 4. Teesalu, T. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 16157-62. PMID: 19805273 5. Issitt, T. et al. (2019) iScience 11, 205-223. PMID: 30623799
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD304/NRP1 Protein, C-Fc

References

HT070011-SDS-PAGE-1.jpg

Datasheet

Human CD304/NRP1 Recombinant Protein (C-Fc).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

=

Concentration

×

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

×

V₁ (Stock Vol.)

=

Working Solution

C₂ (Working Conc.)

×

V₂ (Working Vol.)

Recommendation

Contact us for custom quotes, bulk requests and any other issues.

Mail: support@abinScience.com

Distributor list

Human CD304/NRP1 Recombinant Protein (C-Fc)