Recombinant Human CD59 Protein, C-Fc (HB615021) expressed in Mammalian Cells, spanning Met1-Glu101. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian cells
Accession
P13987
Protein length
Met1-Glu101
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
37.77 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CD59 glycoprotein is a ~14 kDa protein. Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation. Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore.
1. Farkas, I. et al. (2002) The Journal of physiology 539, 537-45. PMID: 11882685
2. Meri, S. et al. (1990) Immunology 71, 1-9. PMID: 1698710
3. Okada, H. et al. (1989) Biochemical and biophysical research communications 162, 1553-9. PMID: 2475111
4. Davies, A. et al. (1989) The Journal of experimental medicine 170, 637-54. PMID: 2475570
5. Sugita, Y. et al. (1989) Journal of biochemistry 106, 555-7. PMID: 2606909
6. Bodian, DL. et al. (1997) The Journal of experimental medicine 185, 507-16. PMID: 9053451
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