Recombinant Human CD85d/LILRB2/ILT4 Protein, C-His (HV255011) expressed in Mammalian Cells, spanning Met1-Val461. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian Cells
Accession
Q8N423
Protein length
Met1-Val461
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
CD85 antigen-like family member D, Monocyte/macrophage immunoglobulin-like receptor 10, MIR-10, ILT4, LIR-2, ILT-4, Immunoglobulin-like transcript 4, MIR10, CD85d, Leukocyte immunoglobulin-like receptor subfamily B member 2, Leukocyte immunoglobulin-like receptor 2, LIR2, LILRB2
Background
Leukocyte immunoglobulin-like receptor subfamily B member 2 (CD85d/LILRB2) is a ~65 kDa protein. Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Competes with CD8A for binding to class I MHC antigens.
1. Lepin, EJ. et al. (2000) European journal of immunology 30, 3552-61. PMID: 11169396
2. Shiroishi, M. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 8856-61. PMID: 12853576
3. Shiroishi, M. et al. (2006) The Journal of biological chemistry 281, 10439-47. PMID: 16455647
4. Gregori, S. et al. (2010) Blood 116, 935-44. PMID: 20448110
5. Köstlin, N. et al. (2017) European journal of immunology 47, 374-384. PMID: 27859042
6. Chang, CC. et al. (2002) Nature immunology 3, 237-43. PMID: 11875462
8. Fanger, NA. et al. (1998) European journal of immunology 28, 3423-34. PMID: 9842885
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD85d/LILRB2/ILT4 Protein, C-His
Bioactivity
Detects Human CD85d/LILRB2 Antibody (J19.H1) in indirect ELISAs.
Bioactivity
Detected by Research Grade JTX-8064-101 (Cat No.: HV255036) in indirect ELISA.
Bioactivity
Detected by IO-108 (Catalog No: HV255046) in indirect ELISA.
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