ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
28.9 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Ubiquitin-conjugating enzyme E2 R1 (CDC34) is a ~26 kDa protein. E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin.
1. Gonen, H. et al. (1999) The Journal of biological chemistry 274, 14823-30. PMID: 10329681
2. Hoeller, D. et al. (2007) Molecular cell 26, 891-8. PMID: 17588522
3. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
4. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650
5. Sheng, Y. et al. (2012) Molecular & cellular proteomics : MCP 11, 329-41. PMID: 22496338
6. Strack, P. et al. (2000) Oncogene 19, 3529-36. PMID: 10918611
7. Gazdoiu, S. et al. (2007) Molecular and cellular biology 27, 7041-52. PMID: 17698585
8. Charrasse, S. et al. (2000) Oncogene 19, 2986-95. PMID: 10871850
9. Butz, N. et al. (2005) Experimental cell research 303, 482-93. PMID: 15652359
10. Cen, B. et al. (2009) The Journal of biological chemistry 284, 5265-76. PMID: 19112177
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CDC34 Protein, N-His
中文
English
한국어
日本語
Español
Français
Русский
