Human CDK2 Recombinant Protein (C-His) (HB845022)

Name: Human CDK2 Recombinant Protein (C-His)
Brand: abinScience
SKU: HB845022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB845022
Description	Recombinant Human CDK2 Protein, C-His (HB845022) expressed in E. coli, spanning Met1-Leu298. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P24941
Protein length	Met1-Leu298
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.88 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CDK2, p33 protein kinase, Cyclin-dependent kinase 2, CDKN2, Cell division protein kinase 2
Background	Cyclin-dependent kinase 2 (CDK2) is a ~33 kDa protein. Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CABLES1, CTNNB1, CDK2AP2, ERCC6, NBN, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, SUV39H1, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in embryonic stem cells (ESCs). 1. Harbour, JW. et al. (1999) Cell 98, 859-69. PMID: 10499802 2. Luciani, MG. et al. (2000) Journal of molecular biology 300, 503-18. PMID: 10884347 3. Zhao, J. et al. (2000) Genes & development 14, 2283-97. PMID: 10995386 4. Ma, T. et al. (2000) Genes & development 14, 2298-313. PMID: 10995387 5. Okuda, M. et al. (2000) Cell 103, 127-40. PMID: 11051553 6. Garrett, S. et al. (2001) Molecular and cellular biology 21, 88-99. PMID: 11113184 7. Terret, ME. et al. (2003) Development (Cambridge, England) 130, 5169-77. PMID: 12944431 8. Esashi, F. et al. (2005) Nature 434, 598-604. PMID: 15800615 9. Brown, NR. et al. (2007) Cell cycle (Georgetown, Tex.) 6, 1350-9. PMID: 17495531 10. Hydbring, P. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 58-63. PMID: 19966300
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CDK2 Protein, C-His

References

HB845022-SDS-PAGE-1.jpg

Datasheet

Human CDK2 Recombinant Protein (C-His).pdf

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Human CDK2 Recombinant Protein (C-His) (HB845022)

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