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Human CDKL5 Recombinant Protein (N-His) (HD781012)

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Overview
Catalog No.HD781012
Description
Recombinant Human CDKL5 Protein, N-His (HD781012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionO76039
Protein lengthMet1-Ser306
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCDKL5, Cyclin-dependent kinase-like 5, EC:2.7.11.22, STK9, Serine/threonine-protein kinase 9
Background

Cyclin-dependent kinase-like 5 is a ~107 kDa protein. Mediates phosphorylation of MECP2. May regulate ciliogenesis.

1. Mari, F. et al. (2005) Human molecular genetics 14, 1935-46. PMID: 15917271
2. Bertani, I. et al. (2006) The Journal of biological chemistry 281, 32048-56. PMID: 16935860
3. Canning, P. et al. (2018) Cell reports 22, 885-894. PMID: 29420175
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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