Human CDKN1A Recombinant Protein (N-His) (HW549012)

Name: Human CDKN1A Recombinant Protein (N-His)
Brand: abinScience
SKU: HW549012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW549012
Description	Recombinant Human CDKN1A Protein, N-His (HW549012) expressed in E. coli, spanning Met1-Pro164. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P38936
Protein length	Met1-Pro164
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.28 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CDKN1, MDA-6, Cyclin-dependent kinase inhibitor 1, CIP1, WAF1, CDKN1A, p21, PIC1, Melanoma differentiation-associated protein 6, MDA6, SDI1, CDK-interacting protein 1, CAP20
Background	Cyclin-dependent kinase inhibitor 1 (p21/CDKN1A) is a ~18 kDa protein. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. 1. LaBaer, J. et al. (1997) Genes & development 11, 847-62. PMID: 9106657 2. Ducoux, M. et al. (2001) The Journal of biological chemistry 276, 49258-66. PMID: 11595739
Note	For research use only.

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SDS-PAGE for Recombinant Human CDKN1A Protein, N-His

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Datasheet

Human CDKN1A Recombinant Protein (N-His).pdf

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Human CDKN1A Recombinant Protein (N-His) (HW549012)

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