Human CHD1L Recombinant Protein (N-His) (HC778012)

Name: Human CHD1L Recombinant Protein (N-His)
Brand: abinScience
SKU: HC778012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC778012
Description	Recombinant Human CHD1L Protein, N-His (HC778012) expressed in E. coli, spanning Ser716-Pro897. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86WJ1
Protein length	Ser716-Pro897
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.48 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ALC1, Amplified in liver cancer protein 1, CHD1L, Chromodomain-helicase-DNA-binding protein 1-like, EC:3.6.4.-
Background	ATP-dependent chromatin remodeler CHD1L is a ~101 kDa protein. ATP-dependent chromatin remodeler that mediates chromatin-remodeling following DNA damage. Recruited to DNA damage sites through interaction with poly-ADP-ribose: specifically recognizes and binds histones that are poly-ADP-ribosylated on serine residues in response to DNA damage. Poly-ADP-ribose-binding activates the ATP-dependent chromatin remodeler activity, thereby regulating chromatin during DNA repair. Catalyzes nucleosome sliding away from DNA breaks in an ATP-dependent manner. Chromatin remodeling activity promotes PARP2 removal from chromatin. 1. Ahel, D. et al. (2009) Science (New York, N.Y.) 325, 1240-3. PMID: 19661379 2. Lehmann, LC. et al. (2017) Molecular cell 68, 847-859.e7. PMID: 29220652 3. Singh, HR. et al. (2017) Molecular cell 68, 860-871.e7. PMID: 29220653 4. Lehmann, LC. et al. (2020) Cell reports 33, 108529. PMID: 33357431 5. Wang, L. et al. (2021) Nature communications 12, 4057. PMID: 34210977 6. Bacic, L. et al. (2021) eLife 10. PMID: 34486521 7. Mohapatra, J. et al. (2021) eLife 10. PMID: 34874266
Note	For research use only

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Datasheet

Human CHD1L Recombinant Protein (N-His).pdf

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Human CHD1L Recombinant Protein (N-His) (HC778012)

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