Human CHD8 Recombinant Protein (N-His) (HC787012)

Name: Human CHD8 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC787012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC787012
Description	Recombinant Human CHD8 Protein, N-His (HC787012) expressed in E. coli, spanning Ser637-Ser780. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9HCK8
Protein length	Ser637-Ser780
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.82 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ATP-dependent helicase CHD8, CHD-8, CHD8, Chromodomain-helicase-DNA-binding protein 8, EC:3.6.4.-, HELSNF1, Helicase with SNF2 domain 1, KIAA1564
Background	ATP-dependent chromatin remodeler CHD8 is a ~290 kDa protein. ATP-dependent chromatin-remodeling factor, it slides nucleosomes along DNA; nucleosome sliding requires ATP. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. 1. Manning, BJ. et al. (2017) The Journal of biological chemistry 292, 11927-11936. PMID: 28533432
Note	For research use only

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Human CHD8 Recombinant Protein (N-His).pdf

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Human CHD8 Recombinant Protein (N-His) (HC787012)

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