Human CHMP4B Recombinant Protein (N-His-SUMO&C-Strep) (HB565012)

Name: Human CHMP4B Recombinant Protein (N-His-SUMO&C-Strep)
Brand: abinScience
SKU: HB565012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB565012
Description	Recombinant Human CHMP4B Protein, N-His-SUMO&C-Strep (HB565012) expressed in E. coli, spanning Asp121-Met224. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9H444
Protein length	Asp121-Met224
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.32 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; CHMP4b; SNF7 homolog associated with Alix 1; SNF7-2; hSnf7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2; hVps32-2; CHMP4B; C20orf178; SHAX1
Background	Charged multivesicular body protein 4b is a ~24 kDa protein. Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. 1. Katoh, K. et al. (2003) The Journal of biological chemistry 278, 39104-13. PMID: 12860994 2. Hanson, PI. et al. (2008) The Journal of cell biology 180, 389-402. PMID: 18209100 3. Guizetti, J. et al. (2011) Science (New York, N.Y.) 331, 1616-20. PMID: 21310966 4. Vietri, M. et al. (2015) Nature 522, 231-5. PMID: 26040712 5. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413 6. Baeumers, M. et al. (2020) BMC biology 18, 200. PMID: 33349255
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human CHMP4B Protein, N-His-SUMO&C-Strep

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Datasheet

Human CHMP4B Recombinant Protein (N-His-SUMO&C-Strep).pdf

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Human CHMP4B Recombinant Protein (N-His-SUMO&C-Strep) (HB565012)

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