ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
35.89 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
C/EBP zeta, C/EBP-homologous protein, C/EBP-homologous protein 10, CCAAT/enhancer-binding protein homologous protein, CHOP, CHOP-10, CHOP10, DDIT-3, DDIT3, DNA damage-inducible transcript 3 protein, GADD153, Growth arrest and DNA damage-inducible protein GADD153
Background
DNA damage-inducible transcript 3 protein is a ~19 kDa protein. Multifunctional transcription factor in endoplasmic reticulum (ER) stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L.
1. Yamaguchi, H. et al. (2004) The Journal of biological chemistry 279, 45495-502. PMID: 15322075
2. Ohoka, N. et al. (2005) The EMBO journal 24, 1243-55. PMID: 15775988
3. Oliveira, SJ. et al. (2009) PloS one 4, e6618. PMID: 19672300
4. Vij, N. et al. (2008) American journal of respiratory cell and molecular biology 38, 176-84. PMID: 17709599
5. Goodall, JC. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 17698-703. PMID: 20876114
6. Ghosh, AP. et al. (2012) PloS one 7, e39586. PMID: 22761832
7. Su, N. et al. (2008) The Journal of biological chemistry 283, 35106-17. PMID: 18940792
8. Park, SH. et al. (2010) The Journal of biological chemistry 285, 35330-9. PMID: 20829347
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CHOP/GADD153/DDIT3 Protein, N-GST & C-His
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