Human CLCN2 Recombinant Protein (N-His) (HA683012)

Name: Human CLCN2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA683012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA683012
Description	Recombinant Human CLCN2 Protein, N-His (HA683012) expressed in E. coli, spanning Leu568-Gly847. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P51788
Protein length	Leu568-Gly847
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.17 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Chloride channel protein 2; ClC-2; CLCN2
Background	Chloride channel protein 2 is a ~98 kDa protein. Voltage-gated and osmosensitive chloride channel. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Displays inward rectification currents activated upon membrane hyperpolarization and extracellular hypotonicity. Contributes to chloride conductance involved in neuron excitability. 1. Peña-Münzenmayer, G. et al. (2005) Journal of cell science 118, 4243-52. PMID: 16155254 2. Blanz, J. et al. (2007) The Journal of neuroscience : the official journal of the Society for Neuroscience 27, 6581-9. PMID: 17567819 3. Saint-Martin, C. et al. (2009) Human mutation 30, 397-405. PMID: 19191339 4. Stölting, G. et al. (2013) Pflugers Archiv : European journal of physiology 465, 1423-37. PMID: 23632988 5. Scholl, UI. et al. (2018) Nature genetics 50, 349-354. PMID: 29403011 6. Fernandes-Rosa, FL. et al. (2018) Nature genetics 50, 355-361. PMID: 29403012 7. Xu, P. et al. (2023) Human genetics 142, 577-593. PMID: 36964785 8. Xu, M. et al. (2024) eLife 12. PMID: 38345841 9. Jeworutzki, E. et al. (2012) Neuron 73, 951-61. PMID: 22405205
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human CLCN2 Protein, N-His

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Datasheet

Human CLCN2 Recombinant Protein (N-His).pdf

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Human CLCN2 Recombinant Protein (N-His) (HA683012)

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