Human CLTC Recombinant Protein (N-His) (HA410012)

Name: Human CLTC Recombinant Protein (N-His)
Brand: abinScience
SKU: HA410012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA410012
Description	Recombinant Human CLTC Protein, N-His (HA410012) expressed in E. coli, spanning Tyr1128-Asn1420. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q00610
Protein length	Tyr1128-Asn1420
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.40 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Clathrin, Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17, CLTC, CLH17, CLTCL2, KIAA0034
Background	Clathrin heavy chain 1 (CLTC) is a ~191 kDa protein. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. 1. Royle, SJ. et al. (2005) Nature 434, 1152-7. PMID: 15858577 2. Royle, SJ. et al. (2006) Journal of cell science 119, 4071-8. PMID: 16968737 3. Booth, DG. et al. (2011) The EMBO journal 30, 906-19. PMID: 21297582 4. Cheeseman, LP. et al. (2013) Journal of cell science 126, 2102-13. PMID: 23532825 5. Ravikumar, B. et al. (2010) Nature cell biology 12, 747-57. PMID: 20639872
Note	For research use only.

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SDS-PAGE for Recombinant Human CLTC Protein, N-His

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Datasheet

Human CLTC Recombinant Protein (N-His).pdf

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Formula

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Human CLTC Recombinant Protein (N-His) (HA410012)

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