Human CNGA3 Recombinant Protein (N-His) (HB528012)

Name: Human CNGA3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB528012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB528012
Description	Recombinant Human CNGA3 Protein, N-His (HB528012) expressed in E. coli, spanning Phe503-Gln694. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q16281
Protein length	Phe503-Gln694
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.44 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Cyclic nucleotide-gated cation channel alpha-3; Cone photoreceptor cGMP-gated channel subunit alpha; Cyclic nucleotide-gated channel alpha-3; CNG channel alpha-3; CNG-3; CNG3; CNGA3; CNCG3
Background	Cyclic nucleotide-gated channel alpha-3 is a ~78 kDa protein. Pore-forming subunit of the cone cyclic nucleotide-gated channel. Mediates cone photoresponses at bright light converting transient changes in intracellular cGMP levels into electrical signals. In the dark, cGMP levels are high and keep the channel open enabling a steady inward current carried by Na(+) and Ca(2+) ions that leads to membrane depolarization and neurotransmitter release from synaptic terminals. Upon photon absorption cGMP levels decline leading to channel closure and membrane hyperpolarization that ultimately slows neurotransmitter release and signals the presence of light, the end point of the phototransduction cascade. Pore-forming subunit of the gustatory cyclic nucleotide-gated channel. 1. Sundin, OH. et al. (2000) Nature genetics 25, 289-93. PMID: 10888875 2. Peng, C. et al. (2003) The Journal of biological chemistry 278, 34533-40. PMID: 12815043 3. Zheng, X. et al. (2022) Nature structural & molecular biology 29, 40-46. PMID: 34969976 4. Hu, Z. et al. (2023) Nature communications 14, 4284. PMID: 37463923
Note	For research use only

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SDS-PAGE for Recombinant Human CNGA3 Protein, N-His

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Datasheet

Human CNGA3 Recombinant Protein (N-His).pdf

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Human CNGA3 Recombinant Protein (N-His) (HB528012)

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