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Human Collagen XVII/COL17A1 Recombinant Protein (C-His) (HV091092)

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Overview
Catalog No.HV091092
Description
Recombinant Human COL17A1 Protein, C-His (HV091092) expressed in E. coli, spanning Glu490-Asn562. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ9UMD9
Protein lengthGlu490-Asn562
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 35.86 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names120 kDa linear IgA dermatosis antigen, 120 kDa linear IgA disease antigen, 180 kDa bullous pemphigoid antigen 2, 97 kDa LAD antigen, 97 kDa linear IgA bullous dermatosis antigen, 97 kDa linear IgA disease antigen, 97-LAD, BP180, BPAG2, Bullous pemphigoid antigen 2, COL17A1, Collagen alpha-1(XVII) chain, LABD97, LAD-1, Linear IgA bullous disease antigen of 97 kDa, Linear IgA disease antigen 1
Background

Collagen alpha-1(XVII) chain is a ~150 kDa protein. May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.

Note For research use only
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References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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