ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CREB-binding protein is a ~265 kDa protein. Acetylates histones, giving a specific tag for transcriptional activation. Mediates acetylation of histone H3 at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac, respectively). Also acetylates non-histone proteins, like DDX21, FBL, IRF2, MAFG, NCOA3, POLR1E/PAF53 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1.
1. Jin, Q. et al. (2011) The EMBO journal 30, 249-62. PMID: 21131905
2. Das, C. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, E1072-81. PMID: 24616510
3. Chen, H. et al. (1999) Cell 98, 675-86. PMID: 10490106
4. Hung, HL. et al. (2001) The Journal of biological chemistry 276, 10715-21. PMID: 11154691
5. Masumi, A. et al. (2003) The Journal of biological chemistry 278, 25401-7. PMID: 12738767
6. Iioka, T. et al. (2003) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 18, 1419-29. PMID: 12929931
7. Chen, S. et al. (2013) Molecular cell 52, 303-13. PMID: 24207024
8. Song, C. et al. (2017) Genes & development 31, 1370-1381. PMID: 28790157
9. Iyer-Bierhoff, A. et al. (2018) Cell reports 25, 2946-2954.e5. PMID: 30540930
10. Zhou, N. et al. (2022) Cell metabolism 34, 1023-1041.e8. PMID: 35675826
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